SAND FLY TAXONOMY TUTORIAL

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Section III: Examine of Phlebotomine Sand Flies for Leishmania

Dissecting the Salivary Glands

  1. Transfer flies to the tube of soap solution and shake gently;
  2. Pour contents of tube onto a fine-mesh screen stretched over the mouth of a large beaker and rinse with tap water until soap is gone;
  3. Using a dissecting pin, lift the flies from the mesh screen and place them in a small Petri dish containing 1x PBS to rinse;
  4. With the 1cc syringe, apply a drop of PBS onto the glass microscope slide;
  5. Place a freshly washed/rinsed sand fly in the drop of PBS on the microscope slide;

9. Aspirate the glands into a 1cc syringe with PBS and transfer to a small Eppendorf tube for storage.


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