Section II: WRBU Sand Fly Mounting Protocol
Positioning Dissected Parts
After the initial positioning, some parts of the specimen may move out of position before the Euparal can dry. For example, heads can easily rotate to one side shortly after mounting. It is a good idea to re-examine all specimens after mounting is complete to ensure nothing has moved.
When repositioning in tacky euparal do not touch specimens directly or you will risk damaging fragile body parts. Instead, simply manipulate the Euparal that surrounds the specimen to move it back into the correct position.
To manipulate specimen into position:
1. Remove head using probes and place in anterior view. Antenna, palps and mouth parts should be clearly distinguishable.
2. Use a dropper or lifter to place specimen on the slide under a dissecting scope. Remove excess ETH with a fine dropper.
3. Place a drop of Euparal on slide to cover specimen. Use a hook-shaped probe to spread out the edges of the Euparal drop so that it flattens against the slide.