SAND FLY TAXONOMY TUTORIAL

« BACK NEXT »

Section II: WRBU Sand Fly Mounting Protocol

Clearing

Some specimens require clearing before mounting to render important internal features more visible. Various media are used for this but some have found that a 10% lacto-phenol solution (Bioquip) works quite well. Place the specimens in lacto-phenol solution for at least an hour but preferably overnight. Specimens may be stored for long periods of time in the lacto-phenol.

Good success has been achieved in making permanent slide mounts with polyvinyl alcohol (PVA) mountant (Bioquip) or Euparal. Place the specimen on a slide in a few drops of the medium and dissect it by removing the head, one wing, and the posterior (last 3-4) segments of the abdomen, which in the female contain the spermathecae and in the male the external genitalia (claspers).

slide mounting

Slide with whole specimen (Lutzomyia longipalpis) in drops of medium

Photo credit: J Stoffer

slide mounting

Dissected specimen (Lutzomyia longipalpis)

Photo credit: J Stoffer


The Walter Reed Biosystematics Unit | Museum Support Center, MRC-534 | Smithsonian Institution | 4210 Silver Hill Rd. | Suitland, MD 20746-2863 USA | Ph: 301-238-1077; FAX: 301-238-3168
Entomology Branch | Walter Reed Army Institute of Research | 503 Robert Grant Avenue | Silver Spring, MD 20910-7500 USA

WRAIR logo  Smithsonian Institution logo © Smithsonian Institution  | Privacy | Terms of use | Contact WRBU