Section II: WRBU Sand Fly Mounting Protocol
Mounting Medium Options
Various techniques have been use for mounting phlebotomine sand flies. Many workers have found water-soluble chloral hydrate media such as gum chloral or Belese's fluid to be suitable for temporary mounts. While they are easy to use, they are not good for permanent preparations because they may shrink as the water evaporates, become crystallized and discolored. To make such mounts more permanent they can be ringed with fingernail polish to prevent moisture from being absorbed into the medium.
Permanent slide mounts can be made using Canada balsam. When using this medium, it is important to dehydrate the specimens by placing them for at least 5 minutes each in 70% alcohol, 90% alcohol, absolute alcohol and finally in xylene. If dehydration is incomplete the specimen will become clouded in the mountant. If specimens are in 80%-90% alcohol, they can be placed in cellosolve (ethylene glycol monoethyl ether) without having to use absolute alcohol and xylene. From the cellosolve they can be put directly into the Canada balsam mountant on the slide.
Fresh temporary mount
Crystallized and discolored mount
Canada balsam mount