Mosquito Mounting Tutorial — Larval Stage

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Section III: Slide-Mounted Mosquito Adult

Dissection of Salivary Glands

1. Transfer mosquitoes to the tube of soap solution and shake gently;

2. Pour contents of tube onto a fine-mesh screen stretched over the mouth of a large beaker and rinse with tap water until soap is gone;

3. Using a dissecting pin, lift the mosquitoes from the mesh screen and place them in a small Petri dish containing 1x PBS to rinse;

4. With the 1cc syringe, apply a drop of PBS onto the glass microscope slide;

5. Place a freshly washed/rinsed mosquito in the drop of PBS on the microscope slide;

9. Aspirate the glands into a 1cc syringe with PBS and transfer to a small Eppendorf tube for storage.


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