Section III: Slide-Mounted Mosquito Adult
Dissection of Salivary Glands
1. Transfer mosquitoes to the tube of soap solution and shake gently;
2. Pour contents of tube onto a fine-mesh screen stretched over the mouth of a large beaker and rinse with tap water until soap is gone;
3. Using a dissecting pin, lift the mosquitoes from the mesh screen and place them in a small Petri dish containing 1x PBS to rinse;
4. With the 1cc syringe, apply a drop of PBS onto the glass microscope slide;
5. Place a freshly washed/rinsed mosquito in the drop of PBS on the microscope slide;
6. With two dissecting needles (straight one in the left hand and bent one in the right hand), remove the mosquito's legs
7. Steady the fly by piercing the thorax at the base of the wing with the straight pin and holding it against the glass; with the bent pin in the right hand, remove the mosquito's head; when the head is pulled from the body, the salivary glands should come with it and will be visible at the back of the head
8. Tease away the glands with the dissecting pins.
9. Aspirate the glands into a 1cc syringe with PBS and transfer to a small Eppendorf tube for storage.