Section II: WRBU Mosquito Larva Mounting Protocol
Many of the taxonomic characters used to make species-level identifications can only be seen using a compound microscope so mosquito larval specimens should be slide mounted immediately after being cleared. All parts should be positioned not to block any identifying characters. Most important taxonomic characters will be the ..., ... and ....
The Euparal solution must be thick enough to hold the specimen parts in position, but thin enough that it can be readily manipulated. If it is too thick, thin it with Euparal Essence. Euparal Essence may be used instead of alcohol to position the specimen, but be careful — the essence must be wiped away enough so that the Euparal doesn't spread out too much over the slide.
After Euparal has been added, take care to make sure specimen remains positioned and that no air bubbles surface around the specimen. Bubbles and repositioning can be accomplished by using the probes to gently move the specimen or remove the bubbles.
1. Use a diamond tip pencil to inscribe specimen number on a slide and clean with ETH.
2. Use a dropper or lifter to place specimen on the slide under a dissecting scope. Remove excess ETH with a fine dropper.
3. Place a drop of Euparal on slide to cover specimen. Use a hook-shaped probe to spread out the edges of the Euparal drop so that it flattens against the slide.